Part:BBa_K5443013:Experience

Applications of BBa_K5443013

We showed that the ECH-Ppu enzyme from Pseudomonas putida was functional in E. coli as part of a vanillin biosynthetic pathway. This was evidenced by detectable production of vanillin (0.2 ppm), determined by LC-MS analysis of our tested clone pMQ3C-11 (Fig. 1).

As functional ECH-Ppu is essential for vanillin production in this pathway, detectable presence of vanillin shows at least partial success of our construct pMQ3C-11.

Figure 1. LCMS analysis of compounds produced using pMQ3C-11.
The graph shows the detected presence of all 6 tested compounds, validating successful expression of all parts within pMQ3C-11, including ECH-Ppu.